ENTEROCOCCI AND THEIR ABILITY TO FORM A BIOFILM

  • Margita Čanigová Slovak University of Agriculure in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Viera Ducková Slovak University of Agriculure in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Miroslav Kročko Slovak University of Agriculure in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Jana Račková Slovak University of Agriculure in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Jana Bezeková Slovak University of Agriculure in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
Keywords: milk, enterococci, identification, biofilm

Abstract

Number of enterococci determined in raw milk cistern samples was in range of 2.95 to 4.18 log CFU.ml-1 and raw milk samples obtained from storage tanks contained enterococci count in the range of 3.04 to 4.51 log CFU.ml-1. Results of microbiological quality evaluation showed, that count of enterococci increased during cold storage of raw milk. Portion of enterococci from the total microflora of raw milk taken from cistern samples was 0.44 %, otherwise in raw milk samples taken from storage tanks portion of enterococci decreased to 0.38 %. Among enterococci isolates  E. faecalis was the predominat species in tested samples of raw milk from both cistern – 58.1 % and storage tank – 71.7 %. The following species were identified E. faecium, E. group III., E. mundtii, E. casseliflavus. It was found that 38 % E. faecalis isolates were able to form a biofilm.

doi:10.5219/200

References

 

BARBOSA, J., GIBBS, P. A., TEIXEIRA, P. 2010. Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal. In Food Control., vol. 21, 2010, no. 5, p. 651-656. doi:10.1016/j.foodcont.2009.10.002


BIENDO, M., ADJIDE, C., CASTELAIN, S., BELMEKKI, M., ROUSSEAU, F., SLAMA, M., GANRY, O., SCHMIT, J. L., EB, F. 2010. Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France). In International Journal of Microbiology, vol. 2010, no. 1, p. 1-8.doi:10.1155/2010/150464 PMid:21052490


CITAK, S., GUNDOGAN, N., MEND, A. 2006. Occurence, isolation and antibiotic resistance of Enterococcus species isolated from raw milk samples in Turkey. In Milchwissenschaft., vol. 61, 2006, no. 2, p. 150-152.


DI ROSA,  R., CRETI, R., VENDITTI, M., D´AMELIO, R., ARCIOLA, C., MONTANARO, L., BALDASSARRI, L. 2006. Relationship between biofilm formation, the enterococcal surface protein and gelatinase in clinical isolates of Enterococcus faecalis and Enterococcus faecium. In FEMS Micobiology Letters. vol. 256, 2006, no. 1, p. 145-150. doi:10.1111/j.1574-6968.2006.00112.xPMid:16487332


DUCKOVÁ, V., ČANIGOVÁ, M., KROČKO, M. 2009. Enterococcus species isolated from sheep milk and Slovak Bryndza cheese and their antibiotic suspectibility. In Milchwissenschaft., vol. 64, 2009, no. 1, p. 70-74.


FOULQUIE MORENO, M. R., SARATINOPOULUS, P., TSAKALIDOU, E., DE VUYST, L. 2006. The role and application of enterococci in food and health. In International Journal of Systematic Bacteriology., vol.106, 2006, no.1, p. 1-24.


GOMES, B. C., ESTEVES, C. T., PALAZZO, I. C. V., FELIS, G. E., SECHI, L. A., FRANCO, B. D. G. M., DE MARTINIS, E. C. P. 2008. Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. In Food Microbiology., vol 25, 2008, no. 5, p. 668-675. doi.org/10.1016/j.fm.2008.03.008PMid:18541165


KOREŇOVÁ, J., LOPAŠOVSKÁ, J., KUCHTA, T. 2008. Comparison of three microtitre plate-based methods for quantification of biofilm formation ability of bacteria contaminating food technologies. In Journal of Food and Nutrition Research., vol. 47, 2008, no. 2, p. 100-104.


KROČKO, M., ČANIGOVÁ, M., DUCKOVÁ, V. 2007. Porovnanie selektívnych kultivačných médií pre stanovenie počtu enterokokov. In Laboralim 2007: zborník   prednáškových a posterových príspevkov zo XVI. Medzinárodnej konferencie o analytických metódach v potravinárstve v súlade s harmonizáciou legislatívy EÚ v dňoch 7. a 8. februára 2007 v Banskej Bystrici. Bratislava: Slovenská technická univerzita. 2007, ISBN 80-227-2222-7, p. 295-299.


METZGER, S. 2008. Biofilm formation by Enterococcus species of bovine mammary gland and environmental origins. In: The Knowledge bank at OSU, The Ohio State University: Department of Animal Sciences Honors Theses, 2008, pp 11.


MOHAMED, J. A., HUANG, W., NALLAPAREDDY, S. R., TENG, F., MURRAY, B. E. 2004. Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. In Infection and Immunity., vol. 72, 2004, no. 6, p. 3658-3663. doi:10.1128/IAI.72.6.3658-3663.2004PMid:15155680


NECIDOVÁ, L., JANŠTOVÁ, B., KARPÍŠKOVÁ, S., CUPÁKOVÁ, S., DUŠKOVÁ, M., KARPÍŠKOVÁ, R. 2009. Importance of Enterococcus spp. for forming a biofilm. In Czech Journal of Food Science, vol. 27, 2009, no. 2, p. 354-356.


NES, I. F., DIEP, D. B., HOLO, H. 2007. Bacteriocin Diversity in Streptococcus and Enterococcus. In Journal of Bacteriology., vol. 189, 2007, no. 4, p. 1189-1198. doi:10.1128/JB.01254-06 PMid: 17098898


PANFILI, G., FRATIANNI, A., DI CRISCIO, T., GAMMARIELLO, D., SORRENTINO, E. 2008. Influence of microorganisms on retinol isomerization in milk. In Journal of Dairy Research., vol. 75, 2008, no. 1, p. 37-43. doi:10.1017/S0022029907003044PMid:18226309


SERIO, A., CHAVES-LÓPEZ, C., PAPARELLA, A. SUZZI, G. 2010. Evaluation of metabolic activities of enterococci isolated from Pecorino Abruzzese cheese. In International Dairy Journal., vol. 20, 2010,  no. 7, p. 459-464.doi:10.1016/j.idairyj.2010.02.005


STN 56 0100: 1970. Mikrobiologické skúšanie požívatín, predmetov bežnej spotreby a prostredia potravinárskych prevádzok. Bratislava : ÚNMS, 1970. 239 s.


STANDAROVÁ, E., BORKOVCOVÁ, I., DUŠKOVÁ, M., PRIDALOVÁ, H., DRAČKOVÁ, M., VORLOVÁ, L. 2009. Effect of Some Factors on the Biogenic Amines and Polyamines Content in Blue-Veined Cheese Niva. In Czech Journal of Food Science vol. 27, 2009, special issue,  p. 410-413.

 

TENDOLKAR, P. M., BAGHDAYAN, A. S., GILMORE, M. S., SHANKAR, N. 2004. Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. In Infection and Immunity., vol. 72, 2004, no. 10, p. 6032-6039. doi:10.1128/IAI.72.10.6032-6039.2004 PMid:15385507

 

TEIXEIRA, L. M., CARVALHO, M. S., FACKLAM, R. R. 2007. Enterococcus. In Manual of Clinical Microbiology, 9th ed., Murray, P. R., Baron, E. J., Jorgensen, J. H., Landry, M. L., Pfaller, M. A. (eds), ASM Press: Washington D.C. 2007, chap. 30. p. 698-715.


TRIVEDI, K., CUPÁKOVÁ, S., KARPIŠKOVÁ, R. 2011. Virulence factors and antibiotic resistance in enterococci isolated from food-stuffs. In Veterinarni Medicina., vol. 56, 2011, no. 7,  p. 352-357.


TUNCER, Y. 2009. Some technological properties of phenotypically identified enterococci strains isolated from Turkish tulum cheese. In African Journal of Biotechnology., vol. 8, 2009, no. 24, p. 7008-7016.


WEISS, A., DOMIG, K. J., KNEIFEL, W. 2005. Selective media for enumeration of probiotic enterococci. In Food technology and biotechnology, vol. 43, 2005, no. 2, p. 147-155.

 


 

Published
2012-03-05
How to Cite
Čanigová, M., Ducková, V., Kročko, M., Račková, J., & Bezeková, J. (2012). ENTEROCOCCI AND THEIR ABILITY TO FORM A BIOFILM. Potravinarstvo Slovak Journal of Food Sciences, 6(2), 15-18. https://doi.org/10.5219/200