Microscopic fungi isolated from different Slovak grape varieties
Abstract
The aim of this study was to isolate and identify microscopic fungi in different grape samples. We collected 13 grapes varienties samples (9 white and 4 red) from local Slovak winemakers in the end of the September 2017. Used 13 grape samples in this study: Alibernet, Irsai Oliver, Dornfelder, Blue Frankish, Feteasca regala, Green Veltliner, Pálava, Mūller Thurgau, Rhinriesling, Cabernet Savignon, Pinot Blanc, Savignon Blanc and Welschriesling. Microscopic fungi in grape samples were detected on Malt extract agar by spread plate method. The number of microscopic fungi ranged from 2.85 log cfu.g-1 in Cabernet Savignon to 4.83 log cfu.g-1 inFeteasca regala. A total of 627 isolates of microscopic fungi were obtained in this study. The most abundant fungi belonged to genera Alternaria and Penicillium (100% frequency). The high frequency was also detected for Aspergillus (76.92%) and Cladosporium (76.92%) but with lesser relative density. Alternaria sp., Aspergillus niger, Aspergillus sp., Botrytis cinerea, Cladosporium sp., Penicillium expansum, Phoma sp., Rhizopus sp. and Trichoderma sp. species were isolated from grape berries.
References
Abe, L. T., Da Mota, R. V., Lajolo, F. M., Genovese, M. I., 2007. Compostos fenólicos ecapacidade antioxidante de cultivares de uvas Vitis labrusca L. e Vitis vinifera L. Ciência e Tecnologia de Alimentos, vol. 27, no. 2, p. 394-400. https://doi.org/10.1590/s0101-20612007000200032
Abrunhosa L., Paterson R. R., Kozakiewicz Z., Lima N., Venancio A. 2001. Mycotoxin production from fungi isolated from grapes. Letters of Applied Microbiology, vol. 32, no. 4, p. 240-242. https://doi.org/10.1046/j.1472-5x.2001.00897.x
Bau, M., Bragulat, M. R., Abarca, M. L., Minguez, S., Cabañes, F. J., 2005. Ochratoxigenic species from Spanish wine grapes. International Journal of Food Microbiology, vol. 98, no. 2, p. 125-130. https://doi.org/10.1016/j.ijfoodmicro.2004.05.015
Felšöciová, S,. Tančinová, D., Rybárik, Ľ., Mašková, Z. Mycobiota of Slovak wine grapes with emphasis on aspergillus and penicillium species in the south slovak wine region. Potravinarstvo Slovak Journal of Food Sciences, vol. 11, no. 1, p. 496-502. https://dx.doi.org/10.5219/789
Freire, L., Passamani, F. R. F., Thomas, A. B., Nassur, R. S. M. R., Silva, L. M., Paschoal, F. N., Pereira, G. E., Prado, G., Batista, L. R. Influence of physical and chemical characteristics of wine grapes on the incidence of Penicillium and Aspergillus fungi in grapes and ochratoxin A in wines. International Journal of Food Microbiology, vol. 241, p. 181-190. https://doi.org/10.1016/j.ijfoodmicro.2016.10.027
Gautam, A., Sharma, S., Bhadauria, R. 2009. Detection of toxigenic fungi and mycotoxins in medicinally important powdered herbal drugs. The Internet Journal of Microbiology, vol. 7, no. 2, p. 1-8. https://doi.org/10.5580/104b
González, H. H. L., Martinez, E. J., Pacin, A., Resnik, S. L. 1999. Relationship between Fusarium graminearum and Alternaria alternata contamination and deoxinivalenol occurrence on Argentinian durum wheat. Mycopathologia, vol. 144, no. 2, p. 97-102. https://doi.org/10.1023/A:1007020822134
La Guerche, S., Chamont, S., Blancard, D., Dubourdieu, D., Darriet, P. 2005. Origin of (-)-geosmin on grapes: on the complementary action of two fungi, botrytis cinerea and penicillium expansum. Antonie Van Leeuwenhoek, vol. 88, no. 2, p. 131-139. https://doi.org/10.1007/s10482-005-3872-4
Lorenzini, M., Zapparoli, G., 2014a. Characterization and pathogenicity of Alternaria spp. strains associated with grape bunch rot during post-harvest withering. International Journal of Food Microbiology, vol. 186, p. 1-5. https://doi.org/10.1016/j.ijfoodmicro.2014.06.008
Lorenzini, M., Zapparoli, G., 2014b. An isolate morphologically and phylogenetically distinct from Botrytis cinerea obtained from withered grapes possibly represents a new species of Botrytis. Plant Pathology, vol. 63, no. 6, p. 1326-1335. https://doi.org/10.1111/ppa.12216
Lorenzini, M., Zapparoli, G., 2015. Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration. Antonie Van Leeuwenhoek, vol. 108, no. 5, p. 1171-1180. https://doi.org/10.1007/s10482-015-0570-8
Lorenzini, M., Cappello, M. S., Zapparoli, G., 2015. Isolation of Neofusicoccum parvum from withered grapes: strain characterization, pathogenicity and its detrimental effects on passito wine aroma. Journal of Applied Microbiology, vol. 119, no. 5, p. 1335-1344. https://doi.org/10.1111/jam.12931
Lorenzini, M., Zapparoli, G., 2016. Description of taxonomically unde fined Sclerotiniaceae strain from withered rotten-grapes. Antonie Van Leeuwenhoek, vol. 109, no. 2, p. 197-205. https://doi.org/10.1007/s10482-015-0621-1
Lorenzini, M., Cappello, M. S., Logrieco, A., Zapparoli, G. 2016. Polymorphism and phylogenetic species delimitation in filamentous fungi from predominant mycobiota in withered grapes. International Journal of Food Microbiology, vol. 238, p. 56-62. https://doi.org/10.1016/j.ijfoodmicro.2016.08.039 PMid:27591387
Mencarelli, F., Tonutti, P. 2013. Sweet, Reinforced and Fortified Wines. Chichester, UK : John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118569184
Medina, A., Mateo, R., López-Ocaña, L., Valle-Algarra, F. M., Jiménez, M., 2005. Study of spanish grape mycobiota and ochratoxin A production by isolates of Aspergillus tubingensis and other members of Aspergillus section Nigri. Applied and Environmental Microbiology, vol. 71, no. 8, p. 4696-4702. https://doi.org/10.1128/aem.71.8.4696-4702.2005
Narayanasam, P., 2006. Postharvest Pathogens and Disease Management. New York, NY : John Wiley & Sons, Inc., 578 p. ISBN: 9780471751984.
Pitt, J. I., Hocking, A. D. 2009. Fungi and Food Spoilage. 3rd ed. London, UK : Springer. 519 p. ISBN 978-0-387-92206-5. https://doi.org/10.1007/978-0-387-92207-2
Ribereau-Gayon, P., Glories, Y., Maujean, A., Dubourdieu, D. 1998. Handbook of Enology, New York, NY : John Wiley & Sons, Ltd., p. 265-321.
Romero, S. M., Patriarca, A., Fernández Pinto, V., Vaamonde, G., 2007. Effect of water activity and temperature on growth of ochratoxigenic strains of Aspergillus carbonarius isolated from Argentinean dried vine fruits. International Journal of Food Microbiology, vol. 115, no. 2, p. 140-143. https://doi.org/10.1016/j.ijfoodmicro.2006.10.014 PMid:17161486
Rousseaux, S., Diguta, C. F., Radoï-Matei, F., Alexandre, H., Guilloux-Bénatier, M., 2014. Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-fl avors and mycotoxins. Food Microbiology, vol. 38, p. 104-121. https://doi.org/10.1016/j.fm.2013.08.013
Samson, R. A., Frisvad, J. C. 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium: new taxonomic schemes and mycotoxins and other extrolites. Utrecht: Centraalbureau voor Schimmelcultures. 260 p. ISBN 90-70351-53-6.
Samson, R. A., Hoekstra, E. S., Frisvad, J. C., Filtenborg, O. 2002a. Introduction to food- and airborne fungi. 6th revised ed. (with some corrections). Utrecht, Netherland : Centraalbureau voor Schimmelcultures. 389 p. ISBN 90-70351-42-0.
Sage, L., Garon, D., Seigle-Murandi, F., 2004. Fungal microflora and ochratoxin a risk in French vineyards. Journal of Agricultural and Food Chemistry, vol. 52, no. 18, p. 5764-5768. https://doi.org/10.1021/jf049497c
Serra, R., Lourenço, A., Alipio, P., Venâncio, A. 2006. Influence of the region of origin on the mycobiota of grapes with emphasis on Aspergillus and Penicillium species. Mycological Research, vol. 110, no. 8, p. 971-978. https://doi.org/10.1016/j.mycres.2006.05.010
Serra, R., Braga, A., Venâncio, A., 2005. Mycotoxin-producing and other fungi isolated from grapes for wine production, with particular emphasis on ochratoxin A. Research in Microbiology, vol. 156, no. 4, p. 515-521. https://doi.org/10.1016/j.resmic.2004.12.005
Valero, A., Marìn, S., Ramos, A. J., Sanchis, V., 2005. Ochratoxin A-producing species in grapes and sun-dried grapes and their relation to ecophysiological factors. Letters in Applied Microbiology, vol. 41, no. 2, p. 196-201. https://doi.org/10.1111/j.1472-765x.2005.01705.x
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