PERSISTENCE OF L. MONOCYTOGENES VERSUS ADHERENCE ON SOLID SURFACE
Abstract
Listeria monocytogenes is an important food-borne pathogen that frequently persists in food-processing environments despite rigorous sanitation procedures. Specific phenotypes that have been linked to persistence have been previously investigated but no clear association has been demonstrated. In this study we characterised four persistent L. monocytogenes strains isolated from food factories in Austria, Czech Republic, Denmark and Ireland in comparison with four non-persistent counterparts. The L. monocytogenes isolates were analysed for the ability to form biofilm during exponential/stationary growth phase at different temperature at low nutrition stress and adhesion to polystyrene at high-salinity conditions. Persistent and non-persistent strains did not significantly differ in the ability to form biofilm or to adhere to polystyrene microtitre plate.
doi:10.5219/135
References
BORUCKI, M. K., PEPPIN, J. D., WHITE, D., LOGE, F., AND CALL, D. R. 2003. Variation in biofilm formation among strains of Listeria monocytogenes. In Appl. Environ. Microbiol., vol. 69, 2003, p. 7336-7342.
doi:10.1128/AEM.69.12.7336-7342.2003
PMid:14660383
CALY, D., TAKILT, D., LEBRET, V. AND TRESSE, O. 2009. Sodium chloride affects Listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expression. In Lett. Appl. Microbiol., vol. 49, 2009, p. 751-756.
doi:10.1111/j.1472-765X.2009.02735.x
PMid:19793195
CHAVANT, P., MARTINIE, B., MEYLHEUC, T., BELLON-FONTAINE, M. N., HEBRAUD, M. 2002. Listeria monocytogenes LO28: Surface physicochemical properties and ability to form biofilms at different teperatures and growth phases. In Appl. Environ. Microbiol., vol. 68, 2002, p. 728-737.
doi:10.1128/AEM.68.2.728-737.2002
PMid:11823213
DEAN, R. B., DIXON, W. J. 1951. Simplified statistics for small numbers of observations. In Anal. Chem., vol. 23, 1951, p. 636-638.
doi:10.1021/ac60052a025
DJORDJEVIC, D., WIEDMANN, M., MCLANDSBOROUGH, L. A. 2002. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. In Appl. Environ. Microbiol., vol. 68, 2002, p. 2950-2958.
doi:10.1128/AEM.68.6.2950-2958.2002
PMid:12039754
FARBER, J. M., PETERKIN, P. I. 1991. Listeria monocytogenes, a food-borne pathogen. In Microbiol. Rev., vol. 55, 1991, p. 476‑511.)
PMid:1943998
GRINSTEAD, D. 2009. Cleaning and sanitation in food processing environments for the prevention of biofilm formation, and biofilm removal. In Biofilms in the Food and Beverage Industries ed. Fratamico, P. M., Annous B. A. and Gunther IV, N. W. pp. 331-358. Cambridge: Woodhead Publishing.
doi:10.1533/9781845697167.3.331
KALMOKOFF, M. L., AUSTIN, J. W., WAN, X. D., SANDERS, G., BANERJEE, S., FARBER, J. M. 2001. Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions. In J. Appl. Microbiol., vol. 91, 2001, p. 725-734.
doi:10.1046/j.1365-2672.2001.01419.x
PMid:11576310
KOREŇOVÁ, J., LOPAŠOVSKÁ, J., KUCHTA, T. 2009. Biofilm forming bacterial contaminants in small and medium-sized ewes' milk and meat processing enterprises in Slovakia. In J. Food Nutr. Res., vol. 48, 2009, p. 115-120.
LELIEVELD, H. L. M. 2006. Sources of contamination. In Hygiene in Food Processing ed. Lelieveld, H. L. M, Mostert, M.A. and White, B, pp. 61-75. Cambridge: Woodhead Publishing.
LIANOU, A., SOFOS, J. N. 2007. A review of the incidence and transmission of Listeria monocytogenes in ready-to-eat products in retail and food service environments. In J. Food Prot., vol. 70, 2007, p. 2172-2198.
doi:10.1016/j.fm.2006.11.002
PMid:17900099
LUNDÉN, J., AUTIO, T., KORKEALA, H. 2003. Persistent and nonpersistent Listeria monocytogenes contamination in meat and poultry processing plants. In J. Food Prot., vol. 66, 2003, p. 2062-2069.
PMid:14627284
MORETRO, T., LANGSRUD, S. 2004. Listeria monocytogenes: biofilm formation and persistence in food-processing environments. In Biofilms, vol. 1, 2004, p. 107-121.
doi:10.1017/S1479050504001322
RODRIGUES, D. A., ALMEIDA, M. A., TEIXEIRA, P. A., OLIVEIRA, R. T. AND AYEREDO, J. C. 2009. Effect of batch and fed-batch growth modes on biofilm formation by Listeria monocytogenes at different temperatures. In Curr. Microbiol., vol. 59, 2009, p. 457-462.
doi:10.1007/s00284-009-9460-5
PMid:19653035
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).





















