ACS Publications. Most Trusted. Most Cited. Most Read

OR SEARCH CITATIONS

My Activity
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Agric. Food Chem. 2005, 53, 11, 4554-4560
RETURN TO ISSUEPREVArticleNEXT

Application of Relative Quantification TaqMan Real-Time Polymerase Chain Reaction Technology for the Identification and Quantification of Thunnus alalunga and Thunnus albacares

View Author Information
Department of Food Technology, AZTI-Fisheries and Food Technological Institute, Txatxarramendi ugartea z/g, E-48395 Sukarrieta (Bizkaia), Spain
Cite this: J. Agric. Food Chem. 2005, 53, 11, 4554–4560
Publication Date (Web):April 30, 2005
https://doi.org/10.1021/jf0500841
Copyright © 2005 American Chemical Society
RIGHTS & PERMISSIONS
Article Views
501
Altmetric
-
Citations
69
LEARN ABOUT THESE METRICS
Read OnlinePDF (191 KB)
Get e-Alerts
SUBJECTS:

Abstract

A novel one-step methodology based on real-time Polymerase Chain Reaction (PCR) technology has been developed for the identification of two of the most valuable tuna species. Nowadays, species identification of seafood products has a major concern due to the importing to Europe of new species from other countries. To achieve this aim, two specific TaqMan systems were devised to identify Thunnus alalunga and Thunnus albacares. Another system specific to Scombroidei species was devised as a consensus system. In addition, a relative quantification methodology was carried out to quantify T. alalunga and T. albacares in mixtures after the relative amount of the target was compared with the consensus. This relative quantification methodology does not require a known amount of standard, allowing the analysis of many more samples together and saving costs and time. The utilization of real-time PCR does not require sample handling, preventing contamination and resulting in much faster and higher throughput results.

Keywords: Species identification; real-time PCR; DNA; Thunnus albacares; Thunnus alalunga; canned tuna

*

 Corresponding author (telephone +34-94-602 99 00; fax +34-94-687 00 06; e-mail [email protected]).

Cited By

This article is cited by 69 publications.

  1. M. A. Motalib Hossain, Md. Eaqub Ali, Sharmin Sultana, Asing, Sharmin Quazi Bonny, Md. Abdul Kader, and M. Aminur Rahman . Quantitative Tetraplex Real-Time Polymerase Chain Reaction Assay with TaqMan Probes Discriminates Cattle, Buffalo, and Porcine Materials in Food Chain. Journal of Agricultural and Food Chemistry 2017, 65 (19) , 3975-3985. https://doi.org/10.1021/acs.jafc.7b00730
  2. Daline Bojolly, Périne Doyen, Bruno Le Fur, Urania Christaki, Véronique Verrez-Bagnis, and Thierry Grard . Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (Thunnus obesus) and Yellowfin Tuna (Thunnus albacares), in Canned Tuna. Journal of Agricultural and Food Chemistry 2017, 65 (4) , 913-920. https://doi.org/10.1021/acs.jafc.6b04713
  3. M. A. Motalib Hossain, Md. Eaqub Ali, Sharifah Bee Abd Hamid, Asing, Shuhaimi Mustafa, Mohd Nasir Mohd Desa, and I. S. M. Zaidul . Double Gene Targeting Multiplex Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Assay Discriminates Beef, Buffalo, and Pork Substitution in Frankfurter Products. Journal of Agricultural and Food Chemistry 2016, 64 (32) , 6343-6354. https://doi.org/10.1021/acs.jafc.6b02224
  4. Rosalee S. Rasmussen Hellberg, Amanda M. Naaum, Sara M. Handy, Robert H. Hanner, Jonathan R. Deeds, Haile F. Yancy, and Michael T. Morrissey . Interlaboratory Evaluation of a Real-Time Multiplex Polymerase Chain Reaction Method for Identification of Salmon and Trout Species in Commercial Products. Journal of Agricultural and Food Chemistry 2011, 59 (3) , 876-884. https://doi.org/10.1021/jf103241y
  5. A. Sánchez, J. Quinteiro, M. Rey-Mendez, R. I. Perez-Martín and C. G. Sotelo . Identification of European Hake Species (Merluccius merluccius) Using Real-Time PCR. Journal of Agricultural and Food Chemistry 2009, 57 (9) , 3397-3403. https://doi.org/10.1021/jf8036165
  6. Momoko Kitaoka, Nobuko Okamura, Hirofumi Ichinose and Masahiro Goto . Detection of SNPs in Fish DNA: Application of the Fluorogenic Ribonuclease Protection (FRIP) Assay for the Authentication of Food Contents. Journal of Agricultural and Food Chemistry 2008, 56 (15) , 6246-6251. https://doi.org/10.1021/jf800300k
  7. Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Patrizia Simoni, Mario Baraldini, Luca De Laude and Aldo Roda. One-Step Triplex-Polymerase Chain Reaction Assay for the Authentication of Yellowfin (Thunnus albacares), Bigeye (Thunnus obesus), and Skipjack (Katsuwonus pelamis) Tuna DNA from Fresh, Frozen, and Canned Tuna Samples. Journal of Agricultural and Food Chemistry 2007, 55 (19) , 7638-7647. https://doi.org/10.1021/jf070902k
  8. Aditi Negi, P. Lakshmi, K. Praba, R. Meenatchi, Akash Pare. Detection of Food Adulterants in Different Foodstuff. 2021,,, 117-164. https://doi.org/10.1002/9781119792130.ch5
  9. Ane del Rio-Lavín, Elisa Jiménez, Miguel Ángel Pardo. SYBR-Green real-time PCR assay with melting curve analysis for the rapid identification of Mytilus species in food samples. Food Control 2021, 130 , 108257. https://doi.org/10.1016/j.foodcont.2021.108257
  10. Lin Yao, Meng Qu, Yanhua Jiang, Yingying Guo, Na Li, Fengling Li, Zhijun Tan, Lianzhu Wang. The development of genus‐specific and species‐specific real‐time PCR assays for the authentication of P atagonian toothfish and A ntarctic toothfish in commercial seafood products. Journal of the Science of Food and Agriculture 2021, 45 https://doi.org/10.1002/jsfa.11507
  11. Syed Muhammad Kamal Uddin, M. A. Motalib Hossain, Zaira Zaman Chowdhury, Mohd Rafie Johan. Detection and discrimination of seven highly consumed meat species simultaneously in food products using heptaplex PCR-RFLP assay. Journal of Food Composition and Analysis 2021, 100 , 103938. https://doi.org/10.1016/j.jfca.2021.103938
  12. Lin Yao, Hongmei Xin, Meng Qu, Yanhua Jiang, Yingying Guo, Fengling Li, Na Li, Zhijun Tan, Lianzhu Wang. Development of duplex real‐time polymerase chain reaction for simultaneous detection of oilfish‑ and escolar‐derived components. Journal of the Science of Food and Agriculture 2021, 101 (5) , 1792-1799. https://doi.org/10.1002/jsfa.10793
  13. Konstantinos V. Kotsanopoulos, Athanasios Exadactylos, George A. Gkafas, Petros V. Martsikalis, Foteini F. Parlapani, Ioannis S. Boziaris, Ioannis S. Arvanitoyannis. The use of molecular markers in the verification of fish and seafood authenticity and the detection of adulteration. Comprehensive Reviews in Food Science and Food Safety 2021, 20 (2) , 1584-1654. https://doi.org/10.1111/1541-4337.12719
  14. Nana Wang, Ning Han, Runze Tian, Jiliang Chen, Xiaoning Gao, Zhiran Wu, Yuqi Liu, Lili Huang. Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker. Frontiers in Microbiology 2021, 12 https://doi.org/10.3389/fmicb.2021.627785
  15. Eliska Servusova, Zora Piskata. Identification of Selected Tuna Species in Commercial Products. Molecules 2021, 26 (4) , 1137. https://doi.org/10.3390/molecules26041137
  16. Brian Klitgaard Hansen, Gregory Kevin Farrant, Rob Ogden, Emily Humble, Guðbjörg Ólafsdóttir, Dorte Bekkevold, Steen Wilhelm Knudsen, Peter Rask Møller, Einar Eg Nielsen, . From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products. ICES Journal of Marine Science 2020, 77 (7-8) , 2557-2566. https://doi.org/10.1093/icesjms/fsaa115
  17. Gongshuai Song, Kang Chen, Haixing Wang, Mengna Zhang, Xina Yu, Jie Wang, Qing Shen. In situ and real-time authentication of Thunnus species by iKnife rapid evaporative ionization mass spectrometry based lipidomics without sample pretreatment. Food Chemistry 2020, 318 , 126504. https://doi.org/10.1016/j.foodchem.2020.126504
  18. Nelson Colihueque, René Espinoza, Margarita Parraguez. Authentication of Frozen Chilean Blue Mussel (Mytilus chilensis) Commercialized in the Town of Osorno, Southern Chile, Using PCR-RFLP Analysis. Recent Patents on Food, Nutrition & Agriculture 2020, 11 (1) , 49-55. https://doi.org/10.2174/2212798410666181231154406
  19. Ferdaous Boughattas, Bruno Le Fur, Romdhane Karoui. Non-Targeted Identification of Brine Covered Canned Tuna Species Using Front-Face Fluorescence Spectroscopy Combined with Chemometric Tools. Food Analytical Methods 2019, 12 (12) , 2823-2834. https://doi.org/10.1007/s12161-019-01638-w
  20. Mohammad Nasir Uddin Ahamad, M. A. Motalib Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Nina Naquiah Ahmad Nizar, Sharmin Quazi Bonny, Mohd Rafie Johan, Md. Eaqub Ali. Tetraplex real-time PCR with TaqMan probes for discriminatory detection of cat, rabbit, rat and squirrel DNA in food products. European Food Research and Technology 2019, 245 (10) , 2183-2194. https://doi.org/10.1007/s00217-019-03326-9
  21. Véronique Verrez-Bagnis, Carmen G. Sotelo, Rogério Mendes, Helena Silva, Kristina Kappel, Ute Schröder. Methods for Seafood Authenticity Testing in Europe. 2019,,, 2063-2117. https://doi.org/10.1007/978-3-319-78030-6_69
  22. A. Sánchez, J. Quinteiro, J.A. Vázquez, R.I. Perez-Martín, C.G. Sotelo. Comparison of real-time PCR methods for quantification of European hake (Merluccius merluccius) in processed food samples. Food Chemistry 2019, 272 , 279-285. https://doi.org/10.1016/j.foodchem.2018.08.031
  23. Pavel Krčmář, Zora Piskatá, Eliška Servusová. Identification of tuna species Thunnus albacares and Katsuwonus pelamis in canned products by real-time PCR method. Acta Veterinaria Brno 2019, 88 (3) , 323-328. https://doi.org/10.2754/avb201988030323
  24. Je-Hung Kuo, Hsiu-Wei Tsuei, Zih-Ling Jia, Che-Yang Lin, Yuan-Hsin Chang, Bo-Lin Chen, Jung Kuan, Hsu-Yang Lin, Lih-Ching Chiueh, Daniel Yang-Chih Shih, Hwei-Fang Cheng. Identification of Ingredient in Mullet Roe Products by the Real-Time PCR Method. Food Analytical Methods 2018, 11 (4) , 992-1000. https://doi.org/10.1007/s12161-017-1043-7
  25. Véronique Verrez-Bagnis, Carmen G. Sotelo, Rogério Mendes, Helena Silva, Kristina Kappel, Ute Schröder. Methods for Seafood Authenticity Testing in Europe. 2018,,, 1-55. https://doi.org/10.1007/978-3-319-54528-8_69-1
  26. Nazia Kanwal, Aisha Tahir. Single Nucleotide Polymorphism Quantitative Polymerase Chain Reaction Assay. 2018,,, 805-822. https://doi.org/10.1016/B978-0-12-802230-6.00038-2
  27. Zora Piskata, Eliska Pospisilova, Gabriela Borilova. Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue. International Journal of Food Properties 2017, 20 (sup1) , S430-S443. https://doi.org/10.1080/10942912.2017.1297953
  28. Jian Ye, Junli Feng, Zhiyuan Dai, Lu Meng, Yanping Zhang, Xiaona Jiang. Application of Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection of Jumbo Flying Squid Dosidicus gigas (D’Orbigny, 1835). Food Analytical Methods 2017, 10 (5) , 1452-1459. https://doi.org/10.1007/s12161-016-0700-6
  29. Ignacio Ortea, Karola Böhme, Pilar Calo-Mata, Jorge Barros-Velázquez. Molecular Techniques - Genomics and Proteomics. 2017,,, 325-354. https://doi.org/10.1002/9781118810224.ch9
  30. Junli Feng, Zhigang Wu, Xiao Xie, Zhiyuan Dai, Shasha Liu. A real-time polymerase chain reaction method for the identification of four commercially important salmon and trout species. Mitochondrial DNA Part A 2017, 28 (1) , 104-111. https://doi.org/10.3109/19401736.2015.1111346
  31. Ryouichi Higashi, Kay Sakuma, Satoru N. Chiba, Nobuaki Suzuki, Seinen Chow, Yasuko Semba, Hiroaki Okamoto, Kenji Nohara. Species and lineage identification for yellowfin Thunnus albacares and bigeye T. obesus tunas using two independent multiplex PCR assays. Fisheries Science 2016, 82 (6) , 897-904. https://doi.org/10.1007/s12562-016-1027-3
  32. Shasha Liu, Kunhua Xu, Zhigang Wu, Xiao Xie, Junli Feng. Identification of five highly priced tuna species by quantitative real-time polymerase chain reaction. Mitochondrial DNA Part A 2016, 27 (5) , 3270-3279. https://doi.org/10.3109/19401736.2015.1015004
  33. Jian Ye, Junli Feng, Shasha Liu, Yanping Zhang, Xiaona Jiang, Zhiyuan Dai. Identification of four squid species by quantitative real-time polymerase chain reaction. Molecular and Cellular Probes 2016, 30 (1) , 22-29. https://doi.org/10.1016/j.mcp.2016.01.001
  34. Leonor Pereira, Sónia Gomes, Paula Martins-Lopes. Nucleic Acid Sample Preparation for Food Traceability. 2016,,, 195-216. https://doi.org/10.1007/978-1-4939-3185-9_14
  35. M. Espiñeira, F. Lago. Advances in Authenticity Testing for Fish Speciation. 2016,,, 415-440. https://doi.org/10.1016/B978-0-08-100220-9.00015-1
  36. LeeAnn Applewhite, Patrick Larkin, Amanda M. Naaum. Species Identification Using Other Tools. 2016,,, 133-148. https://doi.org/10.1016/B978-0-12-801592-6.00007-3
  37. Karola Böhme, Jorge Barros-Velázquez, Pilar Calo-Mata, José M. Gallardo, Ignacio Ortea. Seafood Authentication using Foodomics. 2015,,, 14-30. https://doi.org/10.1002/9781118930458.ch2
  38. Miriam Aldeguer, María López-Andreo, José A. Gabaldón, Antonio Puyet. Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay. Food Chemistry 2014, 145 , 1086-1091. https://doi.org/10.1016/j.foodchem.2013.09.002
  39. Amaya Velasco, Ana Sánchez, Icíar Martínez, Francisco J. Santaclara, Ricardo I. Pérez-Martín, Carmen G. Sotelo. Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus). Food Chemistry 2013, 141 (3) , 2006-2010. https://doi.org/10.1016/j.foodchem.2013.05.077
  40. Fátima C. Lago, Mercedes Alonso, Juan M. Vieites, Montserrat Espiñeira. Fish and Seafood Authenticity-Species Identification. 2013,,, 419-452. https://doi.org/10.1002/9781118346174.ch16
  41. Filipe Pereira, António Amorim, Barbara van Asch. Genetic and DNA-Based Techniques. 2013,,, 195-220. https://doi.org/10.1016/B978-0-444-59562-1.00008-6
  42. Jijuan CAO, Jingquan LI, Qiuyue ZHENG, Junyi XU. Specific Real-time PCR Detection of Monkfish Ingredients. Food Science and Technology Research 2013, 19 (5) , 759-764. https://doi.org/10.3136/fstr.19.759
  43. Shuangya Chen, Jin Zhang, Weiling Chen, Yongxiang Zhang, Jiahe Wang, Dunming Xu, Yu Zhou. Quick method for grouper species identification using real-time PCR. Food Control 2012, 27 (1) , 108-112. https://doi.org/10.1016/j.foodcont.2012.03.011
  44. Po-Shun Chuang, Meng-I Chen, Jen-Chieh Shiao. Identification of tuna species by a real-time polymerase chain reaction technique. Food Chemistry 2012, 133 (3) , 1055-1061. https://doi.org/10.1016/j.foodchem.2012.01.076
  45. Sayuri TSURUDA, Kouichi AKAKI, Hiroshi HIWAKI, Akira SUZUKI, Hiroshi AKIYAMA. Multiplex Real-Time PCR Assay for Simultaneous Detection of Omphalotus guepiniformis and Lentinula edodes. Bioscience, Biotechnology, and Biochemistry 2012, 76 (7) , 1343-1349. https://doi.org/10.1271/bbb.120090
  46. Marta Prado, Ana Boix, Christoph von Holst. Novel approach for the simultaneous detection of DNA from different fish species based on a nuclear target: quantification potential. Analytical and Bioanalytical Chemistry 2012, 403 (10) , 3041-3050. https://doi.org/10.1007/s00216-012-5852-7
  47. LeeAnn Applewhite, Rosalee Rasmussen, Michael Morrissey. Species Identification of Seafood. 2012,,, 193-219. https://doi.org/10.1002/9781118229491.ch16
  48. Beatriz Herrero, Fátima C. Lago, Juan M. Vieites, Montserrat Espiñeira. Real-time PCR method applied to seafood products for authentication of European sole ( Solea solea ) and differentiation of common substitute species. Food Additives & Contaminants: Part A 2012, 29 (1) , 12-18. https://doi.org/10.1080/19440049.2011.623682
  49. Ick-Hyun Jo, Kyong-Hwan Bang, Young-Chang Kim, Jei-Wan Lee, A-Yeon Seo, Bong-Jae Seong, Hyun-Ho Kim, Dong-Hwi Kim, Seon-Woo Cha, Yong-Gu Cho, Hong-Sig Kim. Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes. Journal of Ginseng Research 2011, 35 (4) , 504-513. https://doi.org/10.5142/jgr.2011.35.4.504
  50. Beatriz Herrero, Fátima C. Lago, Juan M. Vieites, Montserrat Espiñeira. Development of a rapid and simple molecular identification methodology for true sardines (Sardina pilchardus) and false sardines (Sardinella aurita) based on the real-time PCR technique. European Food Research and Technology 2011, 233 (5) , 851-857. https://doi.org/10.1007/s00217-011-1577-7
  51. Beatriz Herrero, Juan M. Vieites, Montserrat Espiñeira. Authentication of Atlantic salmon (Salmo salar) using real-time PCR. Food Chemistry 2011, 127 (3) , 1268-1272. https://doi.org/10.1016/j.foodchem.2011.01.070
  52. Rosalee S. Rasmussen Hellberg, Michael T. Morrissey. Advances in DNA-Based Techniques for the Detection of Seafood Species Substitution on the Commercial Market. JALA: Journal of the Association for Laboratory Automation 2011, 16 (4) , 308-321. https://doi.org/10.1016/j.jala.2010.07.004
  53. Valentina Terio, Pietro Di Pinto, Nicola Decaro, Antonio Parisi, Costantina Desario, Vito Martella, Canio Buonavoglia, Marilia Giuseppina Tantillo. Identification of tuna species in commercial cans by minor groove binder probe real-time polymerase chain reaction analysis of mitochondrial DNA sequences. Molecular and Cellular Probes 2010, 24 (6) , 352-356. https://doi.org/10.1016/j.mcp.2010.07.006
  54. Véronique Verrez-Bagnis. Detection of Adulterations. 2010,,, 603-614. https://doi.org/10.1201/EBK1439848173-23
  55. Antonio Puyet, José Bautista. Detection of Adulterations. 2010,,, 615-640. https://doi.org/10.1201/EBK1439848173-24
  56. Hartmut Rehbein. Aquatic Food. 2010,,, 209-219. https://doi.org/10.1002/9780470637685.ch11
  57. Sayuri TSURUDA, Tomonori SAKAMOTO, Kouichi AKAKI, Hiroshi HIWAKI. Detection of Pacific Cod and Capelin Roes in Alaska Pollack Roe Product by Real-Time PCR Assay. Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2010, 51 (3) , 110-114. https://doi.org/10.3358/shokueishi.51.110
  58. Véronique Verrez-Bagnis. Detection of Adulterations. 2009,,, 675-686. https://doi.org/10.1201/9781420046359-c34
  59. Antonio Puyet, José Bautista. Detection of Adulterations. 2009,,, 687-712. https://doi.org/10.1201/9781420046359-c35
  60. Eva Garcia-Vazquez, Jose L. Horreo, Daniel Campo, Gonzalo Machado-Schiaffino, Iliana Bista, Alexandros Triantafyllidis, Francis Juanes. Mislabeling of Two Commercial North American Hake Species Suggests Underreported Exploitation of Offshore Hake. Transactions of the American Fisheries Society 2009, 138 (4) , 790-796. https://doi.org/10.1577/T08-169.1
  61. Rosalee S. Rasmussen, Michael T. Morrissey. Application of DNA-Based Methods to Identify Fish and Seafood Substitution on the Commercial Market. Comprehensive Reviews in Food Science and Food Safety 2009, 8 (2) , 118-154. https://doi.org/10.1111/j.1541-4337.2009.00073.x
  62. Isabel Mafra, Isabel M. P. L. V. O. Ferreira, M. Beatriz P. P. Oliveira. Food authentication by PCR-based methods. European Food Research and Technology 2008, 227 (3) , 649-665. https://doi.org/10.1007/s00217-007-0782-x
  63. Rosalee S. Rasmussen, Michael T. Morrissey. DNA-Based Methods for the Identification of Commercial Fish and Seafood Species. Comprehensive Reviews in Food Science and Food Safety 2008, 7 (3) , 280-295. https://doi.org/10.1111/j.1541-4337.2008.00046.x
  64. IRENE MARTÍN, TERESA GARCÍA, VIOLETA FAJARDO, MARÍA ROJAS, PABLO E. HERNÁNDEZ, ISABEL GONZÁLEZ, ROSARIO MARTÍN. Real-Time PCR for Quantitative Detection of Bovine Tissues in Food and Feed. Journal of Food Protection 2008, 71 (3) , 564-572. https://doi.org/10.4315/0362-028X-71.3.564
  65. B. Pérez-Villarreal, F. Amárita, C. Bald, M.A. Pardo, I. Sagardia. Validation of traceability in the seafood production chain. 2008,,, 539-566. https://doi.org/10.1533/9781845694586.5.539
  66. I. López-Calleja, I. González, V. Fajardo, I. Martín, P.E. Hernández, T. García, R. Martín. Quantitative detection of goats’ milk in sheep’s milk by real-time PCR. Food Control 2007, 18 (11) , 1466-1473. https://doi.org/10.1016/j.foodcont.2006.11.006
  67. Luis Asensio Gil. PCR-based methods for fish and fishery products authentication. Trends in Food Science & Technology 2007, 18 (11) , 558-566. https://doi.org/10.1016/j.tifs.2007.04.016
  68. Maria Teresa Bottero, Alessandra Dalmasso, Marco Cappelletti, Camillo Secchi, Tiziana Civera. Differentiation of five tuna species by a multiplex primer-extension assay. Journal of Biotechnology 2007, 129 (3) , 575-580. https://doi.org/10.1016/j.jbiotec.2007.01.032
  69. Carlos Infante, Aniela Crespo, Eugenia Zuasti, Marian Ponce, Laura Pérez, Victoria Funes, Gaetano Catanese, Manuel Manchado. PCR-based methodology for the authentication of the Atlantic mackerel Scomber scombrus in commercial canned products. Food Research International 2006, 39 (9) , 1023-1028. https://doi.org/10.1016/j.foodres.2006.02.006

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

Pair your accounts.

Export articles to Mendeley

Get article recommendations from ACS based on references in your Mendeley library.

You’ve supercharged your research process with ACS and Mendeley!

STEP 1:
Click to create an ACS ID

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

MENDELEY PAIRING EXPIRED
Your Mendeley pairing has expired. Please reconnect

This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies. Read the ACS privacy policy.

CONTINUE